Journal
/
Bioengineering
/
Ensemble Force Spektroskopi af Shear Forces
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Ensemble Force Spectroscopy by Shear Forces

Ensemble Force Spektroskopi af Shear Forces

DOI:
10.3791/63741-v

•

07:30 min

•

July 26, 2022

•

, ,
1Department of Chemistry & Biochemistry,Kent State University

Chapters

  • 00:04Introduction
  • 00:46Preparation of the Shear Force Microscope
  • 02:11Unfolding I-Motifs with and without Ligands
  • 03:47Shear Force-Actuated Click Reaction
  • 05:29Results: Real-Time Investigation of the Unfolding of an Ensemble Set of Biomolecular Structures by Shear Force
  • 06:39Conclusion

Summary

Automatic Translation

Automatic Translation

Ensemble force spectroscopy (EFS) er en robust teknik til mekanisk udfoldelse og realtidsføling af et ensemblesæt af biomolekylære strukturer i biofysiske og biosensing felter.

Tags

Keywords: Ensemble Force SpectroscopyShear ForcesHigh ThroughputBiomolecular StructuresMechanical Chemical PropertiesMolecular EnsemblesFluorescence MicroscopeHomogenizerReaction ChamberI-motif DNALigand L2H24OTD’Background Fluorescence

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63741?article_type=v

Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
check_url/63741?article_type=v

Bacterial Immobilization for Imaging by Atomic Force Microscopy
check_url/63741?article_type=v

Biosensor til detektion af antibiotikaresistente Staphylococcus Bakterier
check_url/63741?article_type=v

Investigating Receptor-ligand Systems of the Cellulosome with AFM-based Single-molecule Force Spectroscopy
check_url/63741?article_type=v

Fremstilling af koncentreret, Lipid-baserede Oxygen Microbubble Emulsioner af High Shear Homogenisering og Serial Koncentration
check_url/63741?article_type=v

Måling af Maximum Isometrisk Kraft Dannet med permeabiliseres Skeletal muskelfibre
check_url/63741?article_type=v

Atomic force mikroskopi Imaging og Kraft spektroskopi af understøttede lipiddobbeltlag
check_url/63741?article_type=v

En protokol til brug af Förster Resonance Energy Transfer (FRET) -Force Biosensorer at måle mekaniske kræfter på tværs af Nuclear LINC Complex
check_url/63741?article_type=v

Påvisning af Endotoxin i Nano-formuleringer bruger Limulus Amoebocyte Lysate (LAL) Assays
check_url/63741?article_type=v

3D-udskrivning af in vitro hydrogelmikrobærere ved vekslende viskøs inertial kraftstråle

Read Article