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Effekt af hyaluronsyre 35 kDa på en in vitro-model af for tidlig tarmskade og heling ved hjælp af enteroidafledte monolag
JoVE Journal
Medicine
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JoVE Journal Medicine
Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers
DOI:

09:36 min

July 28, 2022

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Chapters

  • 00:04Introduction
  • 00:46Enteroid Monolayer Formation
  • 03:47Monolayer Treatment and Scratch Wound Assay
  • 06:36Scratch Wound Analysis
  • 07:38Results: Enteroid Generation and Enteroid-Derived Monolayer Wound Healing Assay Procedure, Images, Analysis, and the Effect of HA35 on Wound Healing
  • 08:58Conclusion

Summary

Automatic Translation

Denne protokol beskriver en metode til at etablere og udføre et ridsesårassay på todimensionale (2D) monolag afledt af tredimensionale (3D) enteroider isoleret fra ikke-humant primat ileum.

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