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Chapters
- 00:05Introduction
- 00:50Collecting, Fixing, and Maintaining Samples
- 02:34Surface Analysis of Organs in Fixed and Non-Fixed Material
- 03:18Application of a Fluorochrome Conjugated to Wheat Germ Agglutinin in Fluorescence and Confocal Microscopy
- 04:14Scanning Electron Microscopy of Plant Organs
- 07:30Symbiotic Germination of Orchid Seeds
- 09:41Results: Study of Fungal Colonization in Plant Tissues and Seeds Germinated with Mycorrhizal Fungi
- 11:13Conclusion
Denne protokol har til formål at give detaljerede procedurer til indsamling, fiksering og vedligeholdelse af mycoheterotrofe planteprøver ved anvendelse af forskellige mikroskopiteknikker såsom scanning og transmissionselektronmikroskopi, lys-, konfokal og fluorescensmikroskopi for at studere svampekolonisering i plantevæv og frø spiret med mycorrhizal svampe.
