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演示序列比对以预测跨物种敏感性的工具,用于快速评估蛋白质保存
JoVE Journal
Genetics
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JoVE Journal Genetics
Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
DOI:

16:02 min

February 10, 2023

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Chapters

  • 00:04Introduction
  • 00:56Developing and Running a SeqAPASS Query: Level 1
  • 02:43Developing and Running a SeqAPASS Query: Level 2
  • 03:47Accessing and Understanding the Data: SeqAPASS Level 1 and Level 2
  • 05:54Manipulating Data Settings and Visualizing the Data: SeqAPASS Level 1 and Level 2
  • 07:23Developing and Running a SeqAPASS Analysis: Level 3
  • 08:28Identify Critical Amino Acid Residues through a Literature Search
  • 10:54Visualizing Level 3 SeqAPASS Data and Interpretation of Results
  • 13:45Results: SeqAPASS Level 1, 2, and 3 Analyses of the Conservation of Amino Acid Residues and Level 1 and 2 Analyses of μ Opioid Receptor Conservation Across Taxonomic Groups
  • 15:10Conclusion

Summary

Automatic Translation

在这里,我们提出了一个协议,以利用最新版本的美国环境保护署序列比对来预测跨物种敏感性(SeqAPASS)工具。该协议演示了在线工具的应用,以快速分析蛋白质保存,并提供可定制且易于解释的跨物种化学敏感性预测。

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