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In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis
 

In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis

Article DOI: 10.3791/64003-v • 09:11 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

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Summary
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This protocol describes an apical-out necrotizing enterocolitis (NEC)-in-a-dish model utilizing small intestinal enteroids with reversed polarity, allowing access to the apical surface. We provide an immunofluorescent staining protocol to detect NEC-related epithelial disruption and a method to determine the viability of apical-out enteroids subjected to the NEC-in-a-dish protocol.

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Keywords: In Vitro Apical-out Enteroid Model Necrotizing Enterocolitis Intestinal Inflammation Polarity Reversal 3D Enteroids EDTA PBS DMEM F12 Antibiotic-free Medium Ultra-low Attachment Plate Fixative Triton X-100
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