Journal
/
Medicine
/
体外 坏死性小肠结肠炎的顶端出肠样模型
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis
Please note that all translations are automatically generated. Click here for the English version.

体外 坏死性小肠结肠炎的顶端出肠样模型

DOI:
10.3791/64003-v

•

09:11 min

•

June 08, 2022

•

, ,
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine,University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

Chapters

  • 00:05Introduction
  • 00:58Establishment of Apical-Out Enteroid Necrotizing Enterocolitis (NEC)-in-a-Dish Model
  • 02:44Immunofluorescence Staining and Confocal Microscopy
  • 05:52Apical-Out NEC-in-a-Dish Cell Viability
  • 07:01Results: Establishment of Apical-Out NEC in-a-Dish Model and Assessing Viability of Enteroids
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种顶端坏死性小肠结肠炎（NEC）在培养皿中的模型，该模型利用具有反向极性的小肠类化合物，允许进入顶端表面。我们提供免疫荧光染色方案来检测NEC相关的上皮破坏，并提供一种确定受NEC培养皿中方案影响的顶出类肠蛋白的活力的方法。

Tags

Keywords: In VitroApical-out Enteroid ModelNecrotizing EnterocolitisIntestinal InflammationPolarity Reversal3D EnteroidsEDTAPBSDMEM F12Antibiotic-free MediumUltra-low Attachment PlateFixativeTriton X-100

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64003?article_type=v

在干细胞移植在体外模拟缺血/再灌注模型
check_url/64003?article_type=v

在斑马鱼模型的胎儿酒精暴露评估致畸的变化
check_url/64003?article_type=v

作为脑微血管内皮细胞（CEND）的伸展<em>体外</em创伤性脑损伤模型血脑屏障
check_url/64003?article_type=v

脑Microslices的兴奋性刺激作为<em>在体外</em>中风模型
check_url/64003?article_type=v

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力
check_url/64003?article_type=v

冠状血管生成体模型
check_url/64003?article_type=v

研究血管医疗器械血细胞相容性和宿主细胞活化的体外血流循环模型
check_url/64003?article_type=v

人类心脏肌细胞模型中体温过低预科后心肌保护体外评估
check_url/64003?article_type=v

体外实验性硬化模型：脂质超载介质中的肝细胞培养
check_url/64003?article_type=v

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis

Read Article