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使用单蠕虫数据量化 秀丽隐杆线虫的异质性 - 细菌相互作用
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Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions
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使用单蠕虫数据量化 秀丽隐杆线虫的异质性 - 细菌相互作用

DOI:
10.3791/64027-v

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09:54 min

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July 22, 2022

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, ,
1Department of Biology,Emory University, 2Department of Physics,Emory University

Chapters

  • 00:05Introduction
  • 00:47Preparation and Chemical Permeabilization of Worms
  • 03:41Mechanical Disruption of Individual Worms
  • 07:28Results: Heterogeneous Bacterial Colonization of the C. elegans Intestine
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了在冷麻痹和表面漂白以去除外部细菌后，对单个细菌定植 的秀丽隐杆线虫炎的 96孔破坏。将得到的悬浮液接种在琼脂平板上，以便对大量单个蠕虫中的细菌负荷进行准确的中等通量定量。

Tags

Keywords: Caenorhabditis ElegansBacterial InteractionsSingle-worm DataMechanical Disruption96-well FormatMedium ThroughputQuantificationWorm MeasurementsM9TX01OP50SDS/DTT SolutionChemical PermeabilizationWorm Cuticle

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