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Developmental Biology
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使用二分法减少牛卵母细胞中的线粒体DNA
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
Please note that all translations are automatically generated.
Click here for the English version.
使用二分法减少牛卵母细胞中的线粒体DNA
DOI:
10.3791/64060-v
•
06:15 min
•
July 06, 2022
•
Laura Adams*
1
,
Ying Liu*
1
,
Barbara Durrant
,
Elena Ruggeri
,
Carly Young
,
Rebecca Krisher
,
Irina Polejaeva
1
Department of Animal, Dairy, and Veterinary Sciences
,
Utah State University
,
2
Reproductive Sciences, Beckman Center for Conservation Research
,
San Diego Zoo Wildlife Alliance
,
3
Genus PLC
Chapters
00:04
Introduction
00:47
Centrifugation of the Oocytes
01:51
Preparation of the Oocyte for Bisection
03:00
Bisection of the Oocytes
04:56
Results: Successful and Unsuccessful qPCR Results and Boxplot from Mitochondria-Reduced and Mitochondria-Dense Ooplasts
05:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种方案,以显着减少牛卵母细胞中的线粒体DNA拷贝数(P <0.0001)。该方法利用离心和二分切来大大减少卵母细胞线粒体,并且可以增加在重建的种间体细胞核移植胚胎中发育的机会。
Tags
Keywords: Bisection
Mitochondrial DNA
Bovine Oocyte
Cloning
Heteroplasmy
Centrifugation
Zonae Pellucidae
Pronase
CBT20
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