/

/

啮齿动物心脏中的病毒转基因表达和心律失常风险的评估

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

Please note that all translations are automatically generated. Click here for the English version.

啮齿动物心脏中的病毒转基因表达和心律失常风险的评估

DOI:

10.3791/64073-v

•

05:15 min

•

July 27, 2022

•

Aizhu Lu², Jerry Wang², Ying Xia², Ruonan Gu, Kyoung-Han Kim², Erin E. Mulvihill³, Darryl R. Davis², Rob S. Beanlands, Wenbin Liang²

¹University of Ottawa Heart Institute, ²Department of Cellular and Molecular Medicine,University of Ottawa, ³Department of Biochemistry, Microbiology and Immunology,University of Ottawa

Chapters

00:05Introduction
00:51Viral Transgene Expression in Rodent Ventricular Tissues
01:37Assessment of Cardiac Arrhythmia Susceptibility
03:47Results: Assessment of Cardiac Susceptibility to Arrhythmias
04:35Conclusion

Summary

Automatic Translation

本协议描述了在超声心动图引导下通过直接心肌内注射病毒在大鼠和小鼠心脏中表达转基因的方法。本文还解释了通过对孤立的朗根多夫灌注的心脏进行程序性电刺激来评估心脏对室性心律失常易感性的方法。

Tags

Keywords: Viral Transgene Expression Ventricular Myocardium Cardiac Arrhythmia Langendorff Perfusion ECG Isoproterenol Programmed Electrical Stimulation Ventricular Tachyarrhythmia

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

有针对性的表达绿色荧光蛋白在头发上使用体外病毒转导的卵泡

光学测绘的Langendorff灌注大鼠心脏

半自动小红桃光学心跳分析

膜片钳和灌注技术研究离子通道的表达爪蟾卵母细胞

Respirometric皂甙通透心脏纤维的氧化磷酸化的评估

在体外重组活跃T。谷盗端粒酶

GFP的表达和使用的大里基于图像流式细胞仪的可行性评估

在鼠标提睾肌为外周干细胞迁移分析的微循环活体显微镜

在体外合成在人体细胞修饰蛋白表达的mRNA的感应

一个协议来使用基因集富集分析以确定合适的动物模型转化型研究

Read Article