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Journal / Biology / 一种从人间充质干细胞中分离小细胞外囊泡的简单台式过滤方法…
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一种从人间充质干细胞中分离小细胞外囊泡的简单台式过滤方法
 
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一种从人间充质干细胞中分离小细胞外囊泡的简单台式过滤方法

Article DOI: 10.3791/64106-v • 09:10 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 3, 4, 2, 5, 4, 1, 6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University

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该协议演示了如何使用简单的实验室规模台式设置分离人脐带衍生间充质干细胞的小细胞外囊泡（hUC-MSC-sEVs）。采用纳米颗粒示踪分析、BCA蛋白测定、蛋白质印迹和透射电子显微镜分别对分离的hUC-MSC-sEV的粒径分布、蛋白浓度、sEVs标志物和形貌进行了表征。

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撤稿，第 184 期，
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