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Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays
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Chapters
- 00:04Introduction
- 00:50Purification of the RNA Polymerase and Preparation of RNA Polymerase Stock
- 03:35In Vitro Transcription with the Incorporation of Radiolabeled Nucleotides
- 05:35Results: Analysis of RpoD Purification and In Vitro Transcription
- 06:43Conclusion
In vitro transcription assays can decipher the mechanisms of transcriptional regulation in Borreliella burgdorferi. This protocol describes the steps to purify B. burgdorferi RNA polymerase and perform in vitro transcription reactions. Experimental approaches using in vitro transcription assays require reliable purification and storage of active RNA polymerase.
