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Oprettelse af et knæled-på-en-chip til modellering af ledsygdomme og test af lægemidler
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1Department of Orthopaedic Surgery,University of Pittsburgh School of Medicine, 2Department of Bioengineering,University of Pittsburgh Swanson School of Engineering, 3Department of Neurobiology,University of Pittsburgh School of Medicine, 4Department of Orthopaedic Surgery,Stanford University, 5Department of Microbiology, Immunology, and Genetics,University of North Texas Health Science Center, 6Department of Bioengineering,Stanford University, 7McGowan Institute for Regenerative Medicine,University of Pittsburgh School of Medicine, 8The Chinese University of Hong Kong
Chapters
- 00:05Introduction
- 00:43Engineering the Tissue Components
- 05:49Establishing the Minijoint Chip
- 08:13Individual Tissue Collection
- 09:35Results: Establishment of a Miniature Synovial Joint-Mimicking Microphysiological System
- 12:07Conclusion
Vi leverer detaljerede metoder til generering af fire typer væv fra humane mesenkymale stamceller, som bruges til at rekapitulere brusk, knogle, fedtpude og synovium i det menneskelige knæled. Disse fire væv er integreret i en tilpasset bioreaktor og forbundet via mikrofluidik, hvilket genererer et knæled-på-en-chip.
