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Creación de una articulación de rodilla en un chip para modelar enfermedades articulares y probar medicamentos
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1Department of Orthopaedic Surgery,University of Pittsburgh School of Medicine, 2Department of Bioengineering,University of Pittsburgh Swanson School of Engineering, 3Department of Neurobiology,University of Pittsburgh School of Medicine, 4Department of Orthopaedic Surgery,Stanford University, 5Department of Microbiology, Immunology, and Genetics,University of North Texas Health Science Center, 6Department of Bioengineering,Stanford University, 7McGowan Institute for Regenerative Medicine,University of Pittsburgh School of Medicine, 8The Chinese University of Hong Kong
Chapters
- 00:05Introduction
- 00:43Engineering the Tissue Components
- 05:49Establishing the Minijoint Chip
- 08:13Individual Tissue Collection
- 09:35Results: Establishment of a Miniature Synovial Joint-Mimicking Microphysiological System
- 12:07Conclusion
Proporcionamos métodos detallados para generar cuatro tipos de tejidos a partir de células madre mesenquimales humanas, que se utilizan para recapitular el cartílago, el hueso, la almohadilla de grasa y la membrana sinovial en la articulación de la rodilla humana. Estos cuatro tejidos se integran en un biorreactor personalizado y se conectan a través de microfluídica, generando así una articulación de rodilla en un chip.
