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Developmental Biology

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JoVE Journal Developmental Biology
En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
 

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Article DOI: 10.3791/64207-v • 08:57 min • July 27th, 2022
July 27th, 2022 •
1,2, 1,2, 1,2
1Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Ciber de Enfermedades Cardiovasculares

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Summary
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Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

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Keywords: En Face Endocardial Cushion Planar Morphogenesis Mouse Embryos Primitive Endocardium Valve Development Planar Cell Polarity Cardio Development Genotyping Fixation Immunostaining
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