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大豆共生结节多聚体纯化
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal Developmental Biology
Polysome Purification from Soybean Symbiotic Nodules
DOI:

07:02 min

July 01, 2022

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Chapters

  • 00:05Introduction
  • 00:36Preparation of Cytosolic Extracts and Sucrose Cushions
  • 02:09Polysome Purification
  • 03:05RNA Extraction, Quality Control, and Precipitation
  • 05:24Results: Quality Assessment of the TOTAL and PAR Fractions
  • 06:24Conclusion

Summary

Automatic Translation

该协议描述了从完整的大豆结节中纯化真核多体的方法。测序后,基因表达分析的标准管道可用于鉴定转录组和翻译组水平的差异表达基因。

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