Journal
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Polysomrensning fra sojabønne symbiotiske knuder
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal Developmental Biology
Polysome Purification from Soybean Symbiotic Nodules
DOI:

07:02 min

July 01, 2022

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Chapters

  • 00:05Introduction
  • 00:36Preparation of Cytosolic Extracts and Sucrose Cushions
  • 02:09Polysome Purification
  • 03:05RNA Extraction, Quality Control, and Precipitation
  • 05:24Results: Quality Assessment of the TOTAL and PAR Fractions
  • 06:24Conclusion

Summary

Automatic Translation

Denne protokol beskriver en metode til eukaryot polysomrensning fra intakte sojabønneknuder. Efter sekventering kan standardrørledninger til genekspressionsanalyse bruges til at identificere differentielt udtrykte gener på transkriptom- og translatomniveauerne.

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