JoVE Journal
Cancer Research
Cancer Research
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Summary
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该协议描述了从小鼠胸腺中分离双阴性胸腺细胞,然后在δ样4表达骨髓基质细胞系共培养系统(OP9-DL4)上进行逆转录病毒转导和共培养以进行进一步的功能分析。