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使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染 体外 转录的mRNA
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Immunology and Infection
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JoVE Journal Immunology and Infection
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
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使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染 体外 转录的mRNA

DOI:
10.3791/64288-v

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10:16 min

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August 17, 2022

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, , , , , , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Department of Pediatrics,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Chapters

  • 00:05Introduction
  • 01:19In Vitro Transcription (IVT) of Chemically Modified mRNA
  • 02:55Generation of IVT mRNA/PP-sNps
  • 05:22Preparation of the Cells for Transfection and Transfection of the Cultured Cells
  • 07:04Analysis of Cell Transfection Efficacy Using Metridia Luciferase (MetLuc) Assay
  • 07:53Results: Analyzing the Transfection Efficiency of MetLuc-mRNA/PP-sNp
  • 09:12Conclusion

Summary

Automatic Translation

Automatic Translation

使用微流体混合装置开发自组装肽 - 泊洛沙明纳米颗粒（PP-sNp）以封装和递送 体外 转录的信使RNA。所描述的mRNA / PP-sNp可以在 体外有效地转染培养的细胞。

Tags

MRNA TransfectionPeptide-poloxamine NanoparticlesMucosal MRNA VaccineLung CancerCystic FibrosisIn Vitro TranscriptionCapped MRNAAgarose Gel ElectrophoresisPoloxamine 704Synthetic Peptide

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