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Chemistry
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构建环状细胞穿透肽以增强生物屏障的穿透力
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JoVE Journal
Chemistry
Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
Please note that all translations are automatically generated.
Click here for the English version.
构建环状细胞穿透肽以增强生物屏障的穿透力
DOI:
10.3791/64293-v
•
10:12 min
•
September 19, 2022
•
Dan Fang
,
Rui Wang
,
Xinyi Yu
,
Yuan Tian
1
Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering
,
Southwest Jiaotong University
Chapters
00:04
Introduction
01:10
Equipment Preparation
01:44
Synthesis of FITC‐Labeled Linear R8 Peptide and FITC‐Labeled Stapled R8 Peptide
05:49
Exploration of the Cell‐to‐Cell Penetration of the Peptides Using Transwell Models
07:06
Results: Synthesis of FITC‐R8 and FITC‐sR8‐4 Using Solid‐Phase Peptide Synthesis and Evaluation of Their Trans‐Barrier Permeability
09:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了具有芳香交联的环细胞穿透肽的合成以及它们跨生物屏障的渗透性的评估。
Tags
Cyclic Cell-penetrating Peptides
Biological Barriers
Cancer Treatment
Molecular Transporters
Drug Delivery
Peptide Cyclization
Aromatic Cross-links
Hydrophobicity
Manual Peptide Synthesis
Fmoc
HATU
DIPEA
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