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肠系膜上动脉闭塞的肠缺血再灌注损伤的啮齿动物模型
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Rodent Model of Intestinal Ischemia-Reperfusion Injury via Occlusion of the Superior Mesenteric Artery
Please note that all translations are automatically generated.
Click here for the English version.
肠系膜上动脉闭塞的肠缺血再灌注损伤的啮齿动物模型
DOI:
10.3791/64314-v
•
06:29 min
•
October 20, 2023
•
Lucie Henein
2
,
Randy Clevenger
,
Karen Keeran
,
Lauren Brinster
1
Animal Surgery and Resources Core, National Heart, Lung, and Blood Institute
,
National Institutes of Health
,
2
College of Veterinary Medicine
,
Mississippi State University
,
3
Division of Veterinary Resources, Office of Research Services
,
National Institutes of Health
Chapters
00:00
Introduction
00:05
Surgical Setup and Animal Preparation
01:29
Surgery and Ischemia
03:56
Tissue Collection and Processing for Histology
05:47
Results: Post-IRI Analysis of Small Intestine
Summary
Automatic Translation
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Automatic Translation
我们描述了如何生成啮齿动物肠缺血再灌注损伤 (IRI) 的广泛使用的手术模型。该手术包括闭塞肠系膜上动脉,然后恢复血流。该模型可用于调查兽医和人类医学中肠道 IRI 闭塞原因的研究。
Tags
Rodent Model
Intestinal Ischemia-reperfusion Injury
Superior Mesenteric Artery Occlusion
Anesthesia
Buprenorphine
Heparin
Abdominal Incision
Superior Mesenteric Artery
Ischemia
Reperfusion
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