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Bioengineering
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基于原子力显微镜的人关节软骨外植体微压痕研究的实际问题
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Bioengineering
Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants
Please note that all translations are automatically generated.
Click here for the English version.
基于原子力显微镜的人关节软骨外植体微压痕研究的实际问题
DOI:
10.3791/64371-v
•
08:06 min
•
October 28, 2022
•
Cyril Daniel
,
Dorothea Alexander
,
Felix Umrath
2
,
Marina Danalache
1
Laboratory of Cell Biology, Department of Orthopaedic Surgery
,
University Hospital Tübingen
,
2
Department of Oral and Maxillofacial Surgery
,
University Hospital Tübingen
Chapters
00:04
Introduction
00:42
Tissue Processing and Generation of Cartilage Discs
01:14
Cartilage Disc Staining and Sorting
02:43
Biomechanical Approach of Cartilage Explants
06:11
Results: AFM Measurements of Cartilage Discs Based on Cellular Pattern Organization
07:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种循序渐进的方法来识别和解决与原子力显微镜微压痕相关的最常见问题。我们举例说明了以不同程度的骨关节炎驱动的变性为特征的天然人类关节软骨外植体的新问题。
Tags
Articular Cartilage
Osteoarthritis
Micro-indentation
Atomic Force Microscopy
Cartilage Explants
Biomechanical Properties
In Vitro Model
Cartilage Disc
Fluorescence Microscopy
AFM Calibration
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