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基于原子力显微镜的人关节软骨外植体微压痕研究的实际问题

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

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基于原子力显微镜的人关节软骨外植体微压痕研究的实际问题

DOI:

10.3791/64371-v

•

08:06 min

•

October 28, 2022

•

Cyril Daniel, Dorothea Alexander, Felix Umrath², Marina Danalache

¹Laboratory of Cell Biology, Department of Orthopaedic Surgery,University Hospital Tübingen, ²Department of Oral and Maxillofacial Surgery,University Hospital Tübingen

Chapters

00:04Introduction
00:42Tissue Processing and Generation of Cartilage Discs
01:14Cartilage Disc Staining and Sorting
02:43Biomechanical Approach of Cartilage Explants
06:11Results: AFM Measurements of Cartilage Discs Based on Cellular Pattern Organization
07:25Conclusion

Summary

Automatic Translation

我们提出了一种循序渐进的方法来识别和解决与原子力显微镜微压痕相关的最常见问题。我们举例说明了以不同程度的骨关节炎驱动的变性为特征的天然人类关节软骨外植体的新问题。

Tags

Articular Cartilage Osteoarthritis Micro-indentation Atomic Force Microscopy Cartilage Explants Biomechanical Properties In Vitro Model Cartilage Disc Fluorescence Microscopy AFM Calibration

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