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氏疏螺旋体复合体和回归热疏螺旋体的栽培方法
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
DOI:

10:37 min

November 25, 2022

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Chapters

  • 00:04Introduction
  • 01:39Preparation of Complete Barbour-Stoenner-Kelly (BSK)-II Medium
  • 02:48Culturing Borrelia with or Without Antibiotics and Long-Term Storage
  • 05:48Isolation of Spirochetes from Environmental or Clinical Sources
  • 07:30Isolation of Monoclonal Populations of B. burgdorferi Sensu Lato Spirochetes and B. miyamotoi from Liquid Cultures by Cultivation on Solid Media
  • 08:39Results: Culturing of Diverse Borrelia Spirochetes
  • 09:23Conclusion

Summary

Automatic Translation

体外培养是检测活细菌存在的直接方法。该协议描述了各种螺旋疏螺旋体的培养方法,包括伯疏螺旋体复合体,回归热疏螺旋体属和宫本疏螺旋体的培养方法。 这些物种挑剔且生长缓慢,但可以养殖。

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