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Journal
/
Immunology and Infection
/
伯
氏疏螺旋体复合体和回归热
疏螺旋
体的栽培方法
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal
Immunology and Infection
Cultivation Methods of Spirochetes from
Borrelia burgdorferi
Sensu Lato Complex and Relapsing Fever
Borrelia
Please note that all translations are automatically generated.
Click here for the English version.
伯
氏疏螺旋体复合体和回归热
疏螺旋
体的栽培方法
DOI:
10.3791/64431-v
•
10:37 min
•
November 25, 2022
•
Anne Berthold*
1
,
Marie-Line Faucillion*
2
,
Ingela Nilsson
,
Maryna Golovchenko
,
Vett Lloyd
,
Sven Bergström
,
Natalie Rudenko
1
Department of Biology
,
Mt. Allison University
,
2
Department of Molecular Biology
,
Umeå University
,
3
Biology Centre Czech Academy of Sciences
,
Institute of Parasitology
Chapters
00:04
Introduction
01:39
Preparation of Complete Barbour-Stoenner-Kelly (BSK)-II Medium
02:48
Culturing
Borrelia
with or Without Antibiotics and Long-Term Storage
05:48
Isolation of
Spirochetes
from Environmental or Clinical Sources
07:30
Isolation of Monoclonal Populations of
B. burgdorferi
Sensu Lato Spirochetes and
B. miyamotoi
from Liquid Cultures by Cultivation on Solid Media
08:39
Results: Culturing of Diverse
Borrelia
Spirochetes
09:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
体外
培养是检测活细菌存在的直接方法。该协议描述了各种螺旋疏螺旋体的培养方法,包括伯
氏
疏螺旋体复合体,回归热疏螺旋体属和宫
本疏螺旋
体的培养方法。
这些物种挑剔且生长缓慢,但可以养殖。
Tags
Spirochetes
Borrelia Burgdorferi
Relapsing Fever Borrelia
Borrelia Miyamotoi
Culture
BSK-II Medium
Bacterial Growth
Rabbit Serum
Gelatin
Anaerobic Atmosphere
Incubation Temperature
Article
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