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Developmental Biology
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评估小鼠卵母细胞中纺锤体组件检查点完整性
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
Please note that all translations are automatically generated.
Click here for the English version.
评估小鼠卵母细胞中纺锤体组件检查点完整性
DOI:
10.3791/64459-v
•
10:09 min
•
September 13, 2022
•
Mansour Aboelenain
2,3
,
Karen Schindler
2
,
Cecilia S. Blengini
2
1
Department of Genetics
,
Rutgers, The State University of New Jersey
,
2
Human Genetics Institute of New Jersey
,
3
Department of Theriogenology, Faculty of Veterinary Medicine
,
Mansoura University
Chapters
00:05
Introduction
00:51
Nocodazole Treatment and Live Imaging
02:41
Monitoring the Pattern of Securin-GFP Degradation During Meiotic Maturation
04:42
Recruitment of MAD2 at Kinetochores by Immunofluorescence During Meiotic Maturation
07:26
Results: Evaluating Spindle Assembly Checkpoint Integrity in Mouse Oocytes
09:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
染色体分离错误是卵母细胞的常见特征。因此,研究纺锤体组装检查点为生产健康卵子所需的机制提供了重要线索。本协议描述了三种互补测定,以评估小鼠卵母细胞中的纺锤体组装检查点完整性。
Tags
Spindle Assembly Checkpoint
Meiosis
Oocyte Aneuploidy
Live Imaging
Immunofluorescence
Nocodazole
Reversine
Securin-GFP
Polar Body Extrusion
Asymmetrical Cytokinesis
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