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肝癌组织来源于小细胞外囊泡的富集方法

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Cancer Research
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JoVE Journal Cancer Research

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

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肝癌组织来源于小细胞外囊泡的富集方法

DOI:

10.3791/64499-v

•

10:33 min

•

February 03, 2023

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Xiaoxing Wang², Jie Chen², Zhengzhe Li², Defa Huang², Xiaomei Yi², Jiyang Wu², Tianyu Zhong²

¹The First School of Clinical Medicine,Gannan Medical University, ²Laboratory Medicine,First Affiliated Hospital of Gannan Medical University

Chapters

00:04Introduction
00:46Tissue Dissociation
02:13Differential Ultracentrifugation
04:42Evaluation of Enrichment Quality
09:04Results: Small Extracellular Vesicles Characterization and Analysis
10:02Conclusion

Summary

Automatic Translation

在这里，我们描述了通过优化的差分超速离心方法富集来自肝癌组织的小细胞外囊泡。

Tags

Keywords: Small Extracellular Vesicles Enrichment Method Differential Ultracentrifugation SEV Isolation PBS Washing SEV Resuspension

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