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从单个骨骼肌中分离静止干细胞群
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles
DOI:

11:35 min

December 09, 2022

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Chapters

  • 00:04Introduction
  • 00:35Isolation of the Gracilis Muscles
  • 01:26Isolation of the Tibialis Anterior (TA) and the Extensor Digitorum Longus (EDL) Muscles
  • 03:17Isolation of the Gastrocnemius (GA) and Soleus Muscles
  • 04:27Isolation of the Triceps Muscles
  • 05:07Isolation of the Masseter Muscles
  • 06:08Isolation of the Diaphragm Muscle
  • 07:20Muscle Digestion to a Single‐Cell Suspension
  • 08:40Results: Purification, Purity Assessment, and Quantitative Measurement of Quiescent Stem Cell Populations from Individual Skeletal Muscles
  • 10:47Conclusion

Summary

Automatic Translation

该协议描述了从小鼠的单个骨骼肌中分离肌肉干细胞和纤维脂肪祖细胞。该方案涉及单肌肉解剖、通过荧光活化细胞分选分离干细胞、通过免疫荧光染色进行纯度评估以及通过 5-乙炔基-2'-脱氧尿苷掺入测定法定量测量 S 期进入。

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