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Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles
 

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles

Article DOI: 10.3791/64557-v • 11:35 min • December 9th, 2022
December 9th, 2022 •
*1, *1, 1, 1, 1
1Department of Biomedicine, Aarhus University
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes the isolation of muscle stem cells and fibro-adipogenic progenitors from individual skeletal muscles in mice. The protocol involves single muscle dissection, stem cell isolation by fluorescence activated cell sorting, purity assessment by immunofluorescence staining, and quantitative measurement of S-phase entry by 5-ethynyl-2´-deoxyuridine incorporation assay.

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Keywords: Stem Cell Skeletal Muscle Isolation Quiescent Regeneration Homeostasis Disease Progression Gracilis Tibialis Anterior (TA) Extensor Digitorum Longus (EDL) Dissection Forceps Scalpel Tendon Tissue Isolation
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