A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Introduction
- 00:35Isolation of the Gracilis Muscles
- 01:26Isolation of the Tibialis Anterior (TA) and the Extensor Digitorum Longus (EDL) Muscles
- 03:17Isolation of the Gastrocnemius (GA) and Soleus Muscles
- 04:27Isolation of the Triceps Muscles
- 05:07Isolation of the Masseter Muscles
- 06:08Isolation of the Diaphragm Muscle
- 07:20Muscle Digestion to a Single‐Cell Suspension
- 08:40Results: Purification, Purity Assessment, and Quantitative Measurement of Quiescent Stem Cell Populations from Individual Skeletal Muscles
- 10:47Conclusion
Detta protokoll beskriver isoleringen av muskelstamceller och fibro-adipogenic progenitorer från enskilda skelettmuskler hos möss. Protokollet innefattar dissektion av enstaka muskler, stamcellsisolering genom fluorescensaktiverad cellsortering, renhetsbedömning genom immunofluorescensfärgning och kvantitativ mätning av S-fasinträde med 5-etynyl-2'-deoxiuridininkorporeringsanalys.
