A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

Carla Guzman-Moreno; Peizhao Zhang; Olivia R. Phillips; Mathias Block; Benjamin J. Glennon; Meghan Holbrook; Lauren Weigand; Cecilia W. Lo; Jiuann-Huey I. Lin

doi:10.3791/64582

Journal

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Developmental Biology

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A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

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Developmental Biology

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JoVE Journal Developmental Biology

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

DOI:

10.3791/64582-v

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08:19 min

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December 16, 2022

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Carla Guzman-Moreno*¹, Peizhao Zhang*², Olivia R. Phillips*¹, Mathias Block*³, Benjamin J. Glennon*¹, Meghan Holbrook, Lauren Weigand, Cecilia W. Lo, Jiuann-Huey I. Lin⁴

¹Department of Developmental Biology,University of Pittsburgh, ²Department of Biological Sciences,University of Pittsburgh, ³Département de Biologie,École Normale Supérieure de Lyon, ⁴Department of Critical Care Medicine,University of Pittsburgh

Chapters

00:05Introduction
01:05Fetal Echocardiogram
01:46Necropsy
02:41Episcopic Confocal Microscopy (ECM)
04:01Three-Dimensional (3D) Reconstruction
06:14Results: Analyzing Cardiac Anomalies Using Echo and ECM
07:19Conclusion

Summary

Automatic Translation

This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

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