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表征胎儿小鼠结构性心脏缺陷的管道

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Developmental Biology

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JoVE Journal Developmental Biology

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

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表征胎儿小鼠结构性心脏缺陷的管道

DOI:

10.3791/64582-v

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08:19 min

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December 16, 2022

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Carla Guzman-Moreno*¹, Peizhao Zhang*², Olivia R. Phillips*¹, Mathias Block*³, Benjamin J. Glennon*¹, Meghan Holbrook, Lauren Weigand, Cecilia W. Lo, Jiuann-Huey I. Lin⁴

¹Department of Developmental Biology,University of Pittsburgh, ²Department of Biological Sciences,University of Pittsburgh, ³Département de Biologie,École Normale Supérieure de Lyon, ⁴Department of Critical Care Medicine,University of Pittsburgh

Chapters

00:05Introduction
01:05Fetal Echocardiogram
01:46Necropsy
02:41Episcopic Confocal Microscopy (ECM)
04:01Three-Dimensional (3D) Reconstruction
06:14Results: Analyzing Cardiac Anomalies Using Echo and ECM
07:19Conclusion

Summary

Automatic Translation

本文详细介绍了使用胎儿超声心动图、尸检和使用会落共聚焦显微镜（ECM）的会射荧光图像捕获（EFIC）然后进行三维（3D）重建的小鼠先天性心脏病（CHD）诊断方法。

Tags

Fetal Mouse Congenital Heart Defects Echocardiography Necropsy Confocal Microscopy Anatomical Changes Structural Birth Defects Ultrasound Color Doppler Sample Preparation Laser Confocal Imaging Image Acquisition

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