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在开顶器官芯片上重建人上皮组织的细胞结构和功能
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
DOI:

09:46 min

February 17, 2023

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Chapters

  • 00:04Introduction
  • 00:52Preparation of Pre‐Gel Solution for the Stroma Equivalent
  • 02:24Surface Micropatterning
  • 03:24Seeding Epithelial Cells on the Stromal Equivalent
  • 04:40Establishing Air‐Liquid Interface (ALI)
  • 05:32Endothelial Cell Seeding
  • 06:24Reconnect the Chip to Flow
  • 07:02Results: Analysis of Human Epithelial Tissues on an Open‐Top Organ‐Chip
  • 08:49Conclusion

Summary

Automatic Translation

本协议描述了开顶器官芯片在成功建立和成熟原代组织(皮肤、肺泡、气道和肠)的全层器官芯片培养物方面的能力和基本培养方式,提供了研究人上皮/间充质和血管生态 位界面的不同功能方面的机会体外。

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