JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Bioengineering
/
在开顶器官芯片上重建人上皮组织的细胞结构和功能
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Bioengineering
Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
Please note that all translations are automatically generated.
Click here for the English version.
在开顶器官芯片上重建人上皮组织的细胞结构和功能
DOI:
10.3791/64633-v
•
09:46 min
•
February 17, 2023
•
Varone Antonio
,
Adya Panchal
,
Magdalena Kasendra
,
Barrile Riccardo
3
1
Merk Sharp & Dohme LLC
,
2
Department of Biomedical Engineering
,
University of Cincinnati
,
3
Center for Stem Cell and Organoid Medicine
,
Cincinnati Children’s Hospital Medical Center
Chapters
00:04
Introduction
00:52
Preparation of Pre‐Gel Solution for the Stroma Equivalent
02:24
Surface Micropatterning
03:24
Seeding Epithelial Cells on the Stromal Equivalent
04:40
Establishing Air‐Liquid Interface (ALI)
05:32
Endothelial Cell Seeding
06:24
Reconnect the Chip to Flow
07:02
Results: Analysis of Human Epithelial Tissues on an Open‐Top Organ‐Chip
08:49
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本协议描述了开顶器官芯片在成功建立和成熟原代组织(皮肤、肺泡、气道和肠)的全层器官芯片培养物方面的能力和基本培养方式,提供了研究人上皮/间充质和血管生态
位界面的不同
功能方面的机会体外。
Tags
Keywords: Open-top Organ-chip
Microfluidic
Microenvironment
Mesenchymal Cells
Collagen Hydrogel
Surface Micropatterning
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/64633?article_type=v
质量流式细胞术:协议,每日调整和执行细胞的样品上一个CyTOF的质量流式细胞仪
check_url/64633?article_type=v
多器官芯片 - 一种微流体平台的长期多组织共培养
check_url/64633?article_type=v
从手术组织小学的人结肠癌细胞的分离和培养直接它们柔软的弹性基底牵引术
check_url/64633?article_type=v
定义人工程心脏组织建设探讨心肌细胞疗法的机理
check_url/64633?article_type=v
提高2D和3D皮肤<em>体外</em>模型使用大分子拥挤
check_url/64633?article_type=v
工程三维上皮组织细胞外基质中嵌入
check_url/64633?article_type=v
人体软组织的生物力学特性采用压痕和拉伸试验
check_url/64633?article_type=v
高维流式细胞术用于解剖种植体组织的免疫功能分析
check_url/64633?article_type=v
在微加工微流体平台中生成简化的三维芯片皮肤模型
check_url/64633?article_type=v
通过心血管磁共振成像定量小鼠心脏左心室功能、心肌劳损和血流动力学力
Read Article