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创新的 3D 打印插入物,旨在实现简单的 2D 和 3D 细胞培养
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Biology
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JoVE Journal
Biology
An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
Please note that all translations are automatically generated.
Click here for the English version.
创新的 3D 打印插入物,旨在实现简单的 2D 和 3D 细胞培养
DOI:
10.3791/64655-v
•
08:17 min
•
January 06, 2023
•
Charlie Colin-Pierre
2,3
,
Oussama El Baraka
,
Laurent Ramont
2,4
,
Stéphane Brézillon
2
1
Laboratoire de Biochimie Médicale et Biologie Moléculaire
,
Université de Reims Champagne-Ardenne
,
2
UMR CNRS 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC
,
Université de Reims Champagne-Ardenne Reims
,
3
BASF Beauty Care Solutions
,
4
Service Biochimie-Pharmacologie-Toxicologie
,
Centre Hospitalier Universitaire (CHU) de Reims
Chapters
00:04
Introduction
00:57
Placement of the Sterilized Insert in the 6‐Well Plates
01:59
Seeding of the Cells
04:21
Implementation of the Co‐Culture, Cell Observation, Counting, and Scraping
05:12
Insert Cleaning for Recycling
05:41
Results: Paracrine Intercellular Communication Between Endothelial Cells and Keratinocytes
07:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
在本文中,提出了一种新设计的3D打印插入物作为共培养模型,并通过研究内皮细胞和角质形成细胞之间的旁分泌细胞间通讯进行了验证。
Tags
3D-printed Insert
2D Cell Culture
3D Cell Culture
Cell Communication
Cell Types
Cell Culture Model
Silicone
Keratinocytes
Human Dermal Microvascular Endothelial Cells
Cell Culture Medium
Trypsin
Centrifugation
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