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创新的 3D 打印插入物,旨在实现简单的 2D 和 3D 细胞培养
JoVE Journal
Biology
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JoVE Journal Biology
An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
DOI:

08:17 min

January 06, 2023

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Chapters

  • 00:04Introduction
  • 00:57Placement of the Sterilized Insert in the 6‐Well Plates
  • 01:59Seeding of the Cells
  • 04:21Implementation of the Co‐Culture, Cell Observation, Counting, and Scraping
  • 05:12Insert Cleaning for Recycling
  • 05:41Results: Paracrine Intercellular Communication Between Endothelial Cells and Keratinocytes
  • 07:38Conclusion

Summary

Automatic Translation

在本文中,提出了一种新设计的3D打印插入物作为共培养模型,并通过研究内皮细胞和角质形成细胞之间的旁分泌细胞间通讯进行了验证。

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