JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
簡単な2Dおよび3D細胞培養を可能にするように設計された革新的な3Dプリントインサート
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
Please note that all translations are automatically generated.
Click here for the English version.
簡単な2Dおよび3D細胞培養を可能にするように設計された革新的な3Dプリントインサート
DOI:
10.3791/64655-v
•
08:17 min
•
January 06, 2023
•
Charlie Colin-Pierre
2,3
,
Oussama El Baraka
,
Laurent Ramont
2,4
,
Stéphane Brézillon
2
1
Laboratoire de Biochimie Médicale et Biologie Moléculaire
,
Université de Reims Champagne-Ardenne
,
2
UMR CNRS 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC
,
Université de Reims Champagne-Ardenne Reims
,
3
BASF Beauty Care Solutions
,
4
Service Biochimie-Pharmacologie-Toxicologie
,
Centre Hospitalier Universitaire (CHU) de Reims
Chapters
00:04
Introduction
00:57
Placement of the Sterilized Insert in the 6‐Well Plates
01:59
Seeding of the Cells
04:21
Implementation of the Co‐Culture, Cell Observation, Counting, and Scraping
05:12
Insert Cleaning for Recycling
05:41
Results: Paracrine Intercellular Communication Between Endothelial Cells and Keratinocytes
07:38
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
この論文では、新しく設計された3Dプリントインサートを共培養のモデルとして提示し、内皮細胞とケラチノサイト間のパラクリン細胞間コミュニケーションの研究を通じて検証します。
Tags
3D-printed Insert
2D Cell Culture
3D Cell Culture
Cell Communication
Cell Types
Cell Culture Model
Silicone
Keratinocytes
Human Dermal Microvascular Endothelial Cells
Cell Culture Medium
Trypsin
Centrifugation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
接着細胞培養用の酸素挿入の作製と動作
エキソソームの定量化とサイズの測定のための革新的方法
細胞間接触の不在で2つの細胞タイプの挿入共培養システムの開発
3Dスフェロイド生産のための効率的で柔軟な細胞凝集法
酸性細胞外 pH 培養系の確立
バンブルビー(
ボンバス
)ハイブ入口のための3Dプリント花粉トラップ
倒立共焦点顕微鏡での上皮組織動態を長期モニタリングするための合理化された生体内イメージングアプローチ
マウスまたは若齢ラットに対するバルク電気鍼治療手術
Application of Live Mitochondria Staining in Cell-Sorting to Purify Hepatocytes Derived from Human Induced Pluripotent Stem Cells
新しい3Dプリントされた直接植物注入(DPI)デバイスを使用した柑橘類植物への化合物の適用
Read Article