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Bioengineering
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肝疾患研究のための末梢血からのヒト肝スフェロイド
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Bioengineering
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JoVE Journal
Bioengineering
Human Liver Spheroids from Peripheral Blood for Liver Disease Studies
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肝疾患研究のための末梢血からのヒト肝スフェロイド
DOI:
10.3791/64703-v
•
09:51 min
•
January 27, 2023
•
Anne-Kathrin Schott
,
Ivan Zipančić
,
Vicente Hernández-Rabaza
,
Zorica A. Becker-Kojić
1
ACA CELL Biotech GmbH
,
2
Departamento Ciencias Biomédicas
,
Universidad Cardenal Herrera-CEU
Chapters
00:05
Introduction
00:58
Preparation of Hepatocyte Differentiation Media
01:27
Culturing Hepatic Cells Differentiated from Blood-Derived Pluripotent Stem Cells (BD-PSCs)
02:14
3D Spheroid Hepatic Differentiation
03:34
Immunofluorescence Analysis of Newly Generated 2D Liver Cell Cultures
04:59
Live Staining of Newly Formed Liver Spheroids
06:01
Examination of Spheroids Using a Fluorescence Microscope
07:32
Results: Differentiation of Human BD-PSCs into Endoderm/Hepatic Progenitor Cells and Hepatocytes
09:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、定常状態の末梢血から単核球を単離した単核球を用いてヒト自家肝スフェロイドを作製する非遺伝的方法を紹介します。
Tags
Keywords: Liver Spheroids
Peripheral Blood
Autologous Sources
Toxicology
Cancer
Drug Discovery
BDPC-derived Hepatocytes
Primary Human Hepatocytes
Regenerative Medicine
Blood-derived Pluripotent Stem Cells
Endodermal Differentiation
Hepatocyte Maturation
Low Attachment Plates
Spheroid Formation
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