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간 질환 연구를 위한 말초 혈액의 인간 간 스페로이드
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Human Liver Spheroids from Peripheral Blood for Liver Disease Studies
DOI:

09:51 min

January 27, 2023

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Chapters

  • 00:05Introduction
  • 00:58Preparation of Hepatocyte Differentiation Media
  • 01:27Culturing Hepatic Cells Differentiated from Blood-Derived Pluripotent Stem Cells (BD-PSCs)
  • 02:143D Spheroid Hepatic Differentiation
  • 03:34Immunofluorescence Analysis of Newly Generated 2D Liver Cell Cultures
  • 04:59Live Staining of Newly Formed Liver Spheroids
  • 06:01Examination of Spheroids Using a Fluorescence Microscope
  • 07:32Results: Differentiation of Human BD-PSCs into Endoderm/Hepatic Progenitor Cells and Hepatocytes
  • 09:11Conclusion

Summary

Automatic Translation

여기에서 우리는 정상 상태 말초 혈액에서 분리된 단핵 세포를 사용하여 인간 자가 간 스페로이드를 생성하는 비유전적 방법을 제시합니다.

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