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Humana leversfäroider från perifert blod för leversjukdomsstudier
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Human Liver Spheroids from Peripheral Blood for Liver Disease Studies
DOI:

09:51 min

January 27, 2023

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Chapters

  • 00:05Introduction
  • 00:58Preparation of Hepatocyte Differentiation Media
  • 01:27Culturing Hepatic Cells Differentiated from Blood-Derived Pluripotent Stem Cells (BD-PSCs)
  • 02:143D Spheroid Hepatic Differentiation
  • 03:34Immunofluorescence Analysis of Newly Generated 2D Liver Cell Cultures
  • 04:59Live Staining of Newly Formed Liver Spheroids
  • 06:01Examination of Spheroids Using a Fluorescence Microscope
  • 07:32Results: Differentiation of Human BD-PSCs into Endoderm/Hepatic Progenitor Cells and Hepatocytes
  • 09:11Conclusion

Summary

Automatic Translation

Här presenterar vi en icke-genetisk metod för att generera humana autologa leversfäroider med hjälp av mononukleära celler isolerade från perifert blod vid steady-state.

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