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Developmental Biology

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JoVE Journal Developmental Biology
Methods for Studying Uterine Contributions to Pregnancy Establishment in an Ovariectomized Mouse Model
 

Methods for Studying Uterine Contributions to Pregnancy Establishment in an Ovariectomized Mouse Model

Article DOI: 10.3791/64763-v • 06:49 min • April 7th, 2023
April 7th, 2023 •
*1,2, *1, 3, 1, *1, *1
1Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology, Monash University, 2Gynaecology Research Centre, Royal Women’s Hospital and Department of Obstetrics and Gynaecology, University of Melbourne, 3The Ritchie Centre, Hudson Institute for Medical Research and Department of Obstetrics and Gynaecology, Monash University
* These authors contributed equally

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Summary
Automatic Translation

Pregnancy establishment is a dynamic process involving complex embryo and uterine crosstalk. The precise contributions of the maternal uterine environment to these processes remain an active area of investigation. Here, detailed protocols are provided to aid in designing in vivo animal models to address these research questions.

Tags

Keywords: Ovariectomy Uterine Contribution Pregnancy Establishment HPG Axis Mouse Model Surgical Techniques Anesthesia Analgesia Sutures Incision Ovarian Fat Pad Uterine Horn
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