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1Herbert Wertheim School Optometry and Vision Science,University of California, Berkeley, 2Department of Ophthalmology,Osaka University Graduate School of Medicine, 3Department of Ophthalmology, Tokyo Medical Center,National Hospital Organization, 4Department of Optometry and Vision Science, School of Optometry,University of Alabama at Birmingham
Chapters
- 00:05Introduction
- 00:59Isolating the Ocular Posterior Eye Cup and the RPE/Choroid/Sclera Complex
- 01:57Isolation of the RPE from the Choroid
- 03:47Results: Assessing the Quality of the Isolated RPE Samples
- 04:29Conclusion
Descrevemos um método simples e eficiente para isolar células do epitélio pigmentado da retina (EPR) de olhos de cobaias pigmentadas jovens. Este procedimento permite o acompanhamento de estudos de biologia molecular do EPR isolado, incluindo análises de expressão gênica.
