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Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

DOI:
10.3791/64868-v

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09:02 min

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September 22, 2023

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, , , ,
1Department of Microbiology,University of Pennsylvania, 2Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine,University of Pennsylvania, 3Otorhinolaryngology-Head and Neck Surgery,University of Pennsylvania, 4Corporal Michael J. Crescenz VA Medical Center

Chapters

  • 00:04Introduction
  • 00:57Infection of Nasal Air‐Liquid Interface (ALI) Cultures
  • 02:53Collection and Quantification of the Shed Virus in Apical Surface Liquid (ASL)
  • 03:49Transepithelial Electrical Resistance (TEER) Measurement
  • 05:25Measuring Cytotoxicity by Lactate Dehydrogenase (LDH) Assay
  • 06:18Results: Characterizing Viral Replication, Host Cell Tropism, and Virus‐Induced Cytotoxicity in Nasal ALI Cultures
  • 08:11Conclusion

Summary

Automatic Translation

Automatic Translation

The nasal epithelium is the primary barrier site encountered by all respiratory pathogens. Here, we outline methods to use primary nasal epithelial cells grown as air-liquid interface (ALI) cultures to characterize human coronavirus-host interactions in a physiologically relevant system.

Tags

Air-liquid InterfaceNasal Epithelial CellsHuman CoronavirusVirus InfectionViral ReplicationHost-virus InteractionsInnate Immune ResponseCytotoxicityOrganoid CultureAirway EpitheliumAsthmaCystic Fibrosis

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