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Cancer Research
/
免疫蛍光アッセイ
による
患者由来卵巣がんオルガノイド中のDNA損傷修復タンパク質の可視化(英語)
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids
via
Immunofluorescence Assays
Please note that all translations are automatically generated.
Click here for the English version.
免疫蛍光アッセイ
による
患者由来卵巣がんオルガノイド中のDNA損傷修復タンパク質の可視化(英語)
DOI:
10.3791/64881-v
•
04:07 min
•
February 24, 2023
•
Lillian van Biljon
,
Bisiayo Fashemi
,
Jeimmy Rodriguez
,
Olivia Graham
,
Amanda Compadre
,
Katherine Fuh
2
,
Dineo Khabele
,
Mary Mullen
1
Washington University in St. Louis
,
2
University of California San Francisco
Chapters
00:04
Introduction
00:50
Immunofluorescence Staining
02:58
Results: Evaluation of DNA Damage Repair Proteins in Organoids
03:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本プロトコルは、患者由来卵巣癌オルガノイドにおけるDNA損傷修復タンパク質を評価するための方法を記載する。ここには、包括的なめっきおよび染色方法、および詳細で客観的な定量手順が含まれています。
Tags
DNA Damage Repair
Ovarian Cancer
Organoids
Immunofluorescence Assay
Biomarkers
PARP Inhibitors
Chemotherapeutic Resistance
3D Cultures
Anti-DNA Damage Repair Protein Antibodies
DAPI
Microscopy
Sample Preparation
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