A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Medical, Oral and Biotechnological Sciences,“G. d’Annunzio” University of Chieti-Pescara, 2Center on Advanced Studies and Technology (CAST),“G. d’Annunzio” University of Chieti-Pescara, 3Department of Medicine and Aging Sciences,University “G. d’Annunzio” of Chieti-Pescara, 4U.O. di Chirurgia Toracica e dei Trapianti di Polmone,Fondazione IRCCS Ca’ Granda – Ospedale Maggiore Policlinico di Milano, 5Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti,Università degli Studi di Milano, 6Department of Pharmaceutical Sciences,Università degli Studi di Milano, 7Unit of Cell and Molecular Biology in Cardiovascular Diseases,Centro Cardiologico Monzino IRCCS
Chapters
- 00:04Introduction
- 00:45Sample Preparation and Enzymatic Digestion
- 01:47Recovery of the Digested Cells and Removal of Non-Endothelial Cells (EC) by Filtration
- 02:32Sedimentation of Cell Clusters and Cell Seeding
- 03:16Results: Purification and Characterization of Freshly Isolated HLMVECs
- 04:30Conclusion
