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改良自体条件血清的生产及其在小鼠角膜上皮中愈合潜力的离体评估

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Medicine

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JoVE Journal Medicine

Production of Modified Autologous Conditioned Serum and Ex Vivo Assessment of Its Healing Potential in Murine Corneal Epithelium

Please note that all translations are automatically generated. Click here for the English version.

改良自体条件血清的生产及其在小鼠角膜上皮中愈合潜力的离体评估

DOI:

10.3791/64911-v

•

03:55 min

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March 24, 2023

•

Chun Hsiung*^1,2, Yu-Ting Liu*^1,2, Chen-Ying Su, Chung-Chuan Hsiung⁵, Kuo-Hsuan Hung^6,7, Lung-Kun Yeh⁶

¹Department of Education,Chang Gung Memorial Hospital Linkou, ²School of Medicine,Chang Gung University, ³Department of Chemical Engineering and Biotechnology,National Taipei University of Technology, ⁴Cell Therapy Center,Tri-Service General Hospital, ⁵Department of General Surgery,Bo-Ai Clinic, ⁶Department of Ophthalmology,Chang Gung Memorial Hospital Linkou, ⁷Institute of Clinical Medicine,National Yang Ming Chiao Tung University

Chapters

00:04Introduction
01:07Preparation for the Modified Autologous Conditioned Serum (mACS) Drops
02:15Results: Assessment of Murine Corneal Wound Healing
03:03Conclusion

Summary

Automatic Translation

本文介绍了一种简化工艺并使自体条件反射血清（ACS）制备成本更低的方案。无需特殊的注射器或表面涂层玻璃珠。此外，改良的ACS（mACS）在鼠眼离体角膜伤口愈合方面比传统自体血清具有竞争优势。

Tags

Modified Autologous Conditioned Serum (mACS)Autologous Serum Wound Healing Corneal Epithelium Ex Vivo Assessment Murine Model Blood-derived Product Anti-inflammation Ocular Surface Disease Cytokine Randomized Controlled Trials

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