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Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis
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JoVE Journal Medicine
Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis

Ferric Chloride-Induced Arterial Thrombosis and Sample Collection for 3D Electron Microscopy Analysis

DOI:
10.3791/64985-v

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03:57 min

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March 17, 2023

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, , , , , , , , , ,
1Department of Molecular and Cellular Biochemistry,University of Kentucky, 2Department of Physiology and Cell Biology,University of Arkansas for Medical Sciences, 3Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering,National Institutes of Health

Chapters

  • 00:05Introduction
  • 00:48Sample Preparation for Serial Block Face Scanning Electron Microscopy Studies Post Ferric Chloride‐Induced Injury
  • 02:47Results: Ferric Chloride‐Mediated Injury to Induce Arterial Thrombosis
  • 03:29Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes how to use a FeCl3-mediated injury to induce arterial thrombosis, and how to collect and prepare arterial injury samples at various stages of thrombosis for electron microscopy analysis.

Tags

Ferric Chloride-Induced Arterial Thrombosis3D Electron Microscopy AnalysisPlateletsHemostatic SystemThrombosisCarotid ArteryIron Chloride InjuryFlow MeasurementsFixativeElectron MicroscopyBlood Flow

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