Scalable Transfection of Maize Mesophyll Protoplasts

Jackson Tonnies; Nicholas A. Mueth; Sayeh Gorjifard; Jonah Chu; Christine Queitsch

doi:10.3791/64991

Journal

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Biology

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Scalable Transfection of Maize Mesophyll Protoplasts

JoVE Journal
Biology
Author Produced

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JoVE Journal Biology

Scalable Transfection of Maize Mesophyll Protoplasts

DOI:

10.3791/64991-v

•

08:38 min

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June 23, 2023

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Jackson Tonnies, Nicholas A. Mueth, Sayeh Gorjifard, Jonah Chu, Christine Queitsch

¹Genome Sciences Department,University of Washington

Chapters

00:04Introduction
00:42Protoplast Isolation
04:11Representative Results
04:26PEG-Mediated Transfection
06:57Representative Results
07:39Conclusion

Summary

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Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.

Scalable Transfection of Maize Mesophyll Protoplasts

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