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Normothermic Ex Vivo Liver Machine Perfusion in Mouse
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JoVE Journal Engineering
Normothermic Ex Vivo Liver Machine Perfusion in Mouse

Normothermic Ex Vivo Liver Machine Perfusion in Mouse

DOI:
10.3791/65363-v

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13:14 min

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September 25, 2023

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, , , ,
1Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery,Jena University Hospital, 2Institute of Pathology,Klinikum Chemnitz GmbH, 3Department of Pathology, Faculty of Veterinary Medicine,Menoufia University

Chapters

  • 00:09Introduction
  • 00:40Preparation
  • 04:33Liver Explanation
  • 09:49Liver and Chamber Connection
  • 10:53Adjust the Flow Rate According to PV Pressure
  • 11:19Representative Results
  • 12:23Conclusion

Summary

Automatic Translation

Automatic Translation

A normothermic ex vivo liver perfusion (NEVLP) system was created for mouse livers. This system requires experience in microsurgery but allows for reproducible perfusion results. The ability to utilize mouse livers facilitates the investigation of molecular pathways to identify novel perfusate additives and enables the execution of experiments focused on organ repair.

Tags

Keywords: NormothermicEx-vivoLiverMachine PerfusionMouseMolecular MechanismsGenetically Modified AnimalsTransgenic MiceThermostatic ControllerPeristaltic PumpOxygenatorBubble TrapPortal VeinSuprahepatic CavaBile DrainageSemi-sterile30G Needle26G CatheterHeparinized SalineOrgan ChamberRoller PumpHeat Exchanger

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