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Journal
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Engineering
/
小鼠常母离
体
肝机灌注
JoVE Journal
Engineering
Author Produced
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JoVE Journal
Engineering
Normothermic
Ex Vivo
Liver Machine Perfusion in Mouse
Please note that all translations are automatically generated.
Click here for the English version.
小鼠常母离
体
肝机灌注
DOI:
10.3791/65363-v
•
13:14 min
•
September 25, 2023
•
Haotian Chen
,
Olaf Dirsch
,
Mohamed Albadry
3
,
Paz Hernandez Ana
,
Uta Dahmen
1
Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery
,
Jena University Hospital
,
2
Institute of Pathology
,
Klinikum Chemnitz GmbH
,
3
Department of Pathology, Faculty of Veterinary Medicine
,
Menoufia University
Chapters
00:09
Introduction
00:40
Preparation
04:33
Liver Explanation
09:49
Liver and Chamber Connection
10:53
Adjust the Flow Rate According to PV Pressure
11:19
Representative Results
12:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为小鼠肝脏创建了常温
离体
肝灌注(NEVLP)系统。该系统需要显微外科手术经验,但允许可重复的灌注结果。利用小鼠肝脏的能力有助于研究分子途径以鉴定新型灌注添加剂,并能够执行专注于器官修复的实验。
Tags
Normothermic
Ex-vivo
Liver
Machine Perfusion
Mouse
Molecular Mechanisms
Genetically Modified Animals
Transgenic Mice
Thermostatic Controller
Peristaltic Pump
Oxygenator
Bubble Trap
Portal Vein
Suprahepatic Cava
Bile Drainage
Semi-sterile
30G Needle
26G Catheter
Heparinized Saline
Organ Chamber
Roller Pump
Heat Exchanger
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