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Journal
/
Neuroscience
/
Simplified and Efficient AAV Production Using HEK293 Suspension Culture
/
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells
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Click here for the English version.
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells
Simplified and Efficient AAV Production Using HEK293 Suspension Culture
DOI:
10.3791/66532-v
•
01:22 min
•
April 26, 2024
•
Kimberly L. Pietersz
,
Paul J.H. Nijhuis
,
Matthijs H.M. Klunder
,
Joëlle van den Herik
,
Barbara Hobo
,
Fred de Winter
,
Joost Verhaagen
2
1
Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience
,
Royal Netherlands Academy of Arts and Sciences (KNAW)
,
2
Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience
,
Vrije Universiteit Amsterdam
Tags
Adeno-associated Virus
AAV
Viral Vector
HEK293 Cells
Suspension Culture
Gene Therapy
Central Nervous System
Neurodegenerative Diseases
Multiple Sclerosis
Parkinson’s Disease
Alzheimer’s Disease
Transfection
Polyethylenimine
TransIt
Ultracentrifugation
Genome Copies
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