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In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
JoVE Journal
Biology
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JoVE Journal Biology
In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
DOI:

08:10 min

March 13, 2008

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Chapters

  • 00:00Title
  • 00:46Introduction
  • 01:15Injecting the Fluorescent Calcium Indicator Dye into the Cortex
  • 05:36Imaging Cortical Calcium Dynamics in vivo
  • 06:57Typical Example of In Vivo Calcium Imaging
  • 07:23Conclusion

Summary

Automatic Translation

To understand network dynamics of microcircuits in the neocortex, it is essential to simultaneously record the activity of a large number of neurons . In-vivo two-photon calcium imaging is the only method that allows one to record the activity of a dense neuronal population with single-cell resolution .

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