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Biology
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芯片尺寸支架的三维细胞培养的微细
JoVE Journal
Biology
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JoVE Journal
Biology
Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
Please note that all translations are automatically generated.
Click here for the English version.
芯片尺寸支架的三维细胞培养的微细
DOI:
10.3791/699-v
•
09:37 min
•
May 12, 2008
•
Stefan Giselbrecht
,
Eric Gottwald
,
Roman Truckenmueller
,
Christina Trautmann
,
Alexander Welle
,
Andreas Guber
,
Volker Saile
,
Thomas Gietzelt
,
Karl-Friedrich Weibezahn
1
Institute for Biological Interfaces
,
Karlsruhe Research Centre
,
2
Institute for BioMedical Technology
,
University of Twente
,
3
Department of Materials Research
,
Institute for Heavy Ion Research
,
4
Institute of Microstructure Technology
,
Karlsruhe Research Centre
,
5
Institute for Micro Process Engineering
,
Karlsruhe Research Centre
Chapters
00:01
Introduction
00:39
Hot Embossing I: Moulding procedure
03:05
Hot Embossing II: Trimming and milling
04:05
Bonding of a microporous membrane to the back of the microcontainers
05:11
SMART: Substrate Modification And Replication by Thermoforming
05:16
SMART Technology I: Irradiation
06:19
SMART Technology II: Microthermoforming
08:27
SMART Technology III: Track Etching
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了两个过程的三维细胞培养的微细多孔聚合物芯片。第一个是与溶剂蒸气的焊接工艺相结合的热压。第二个使用最近开发microthermoforming过程中,结合离子跟踪技术,导致生产的一个显着简化。
Tags
Microfabrication
Chip-sized Scaffolds
Three-dimensional Cell Cultivation
Reproducible Geometry
Constant Quality
Microfabrication Technologies
Manufacturing
Applications
Cell Clusters
Diffusion Path Length
Nutrients
Gases
Apoptosis
Necrosis
Insufficient Nutrient And Gas Supply
Cellular Metabolites
Polymer Chip
CellChip
Microcontainers
Hot Embossing
Micro Injection Moulding
Mass Production
Polymer Microstructures
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