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Journal
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Biology
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哺乳動物細胞をトリプシン処理し、継代培養
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Biology
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JoVE Journal
Biology
Trypsinizing and Subculturing Mammalian Cells
Please note that all translations are automatically generated.
Click here for the English version.
哺乳動物細胞をトリプシン処理し、継代培養
DOI:
10.3791/755-v
•
05:59 min
•
June 12, 2008
•
Richard Ricardo
,
Katy Phelan
1
Molecular Pathology Laboratory Network, Inc
Chapters
00:00
Title
00:37
Introduction
00:56
Trypsinizig and Subculturing Mammalian cells from a Monolayer
04:00
Passaging Cells in Suspension Culture
05:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
細胞がコンフルエントに達するように、彼らは、継代培養や継代する必要があります。このビデオでは、接着および浮遊細胞の両方を継代培養するための手順をデモンストレーションを行います。
Tags
Trypsinizing
Subculturing
Mammalian Cells
Confluency
Passaging
Mitotic Index
Cell Death
Detachment
Primary Culture Vessel
Trypsinization
Mechanical Means
Cell Suspension
Reseeding
Fresh Cultures
Secondary Cultures
Growth
Feeding
Tertiary Cultures
Time Interval
Cell Line
Growth Rate
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