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Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
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Bioengineering
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JoVE Journal Bioengineering
Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

DOI:
10.3791/51880-v

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14:22 min

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September 18, 2016

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, , ,
1Laboratory of Cell Physics,Institut de Science et d’Ingénierie Supramoléculaires (ISIS), CNRS and Université de Strasbourg, 2Development and Stem Cells Program,Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS and Université de Strasbourg

Chapters

  • 00:05Title
  • 02:29Microfabrication of ‘Eggcups’
  • 06:29Introducing Cells into the ‘Eggcups’
  • 09:31Observation of Fixed Cellular Organelles in ‘Eggcups’
  • 11:22Results: ‘Eggcups’ Enable Visualization of Cellular Organelles and Study of Dynamic Cell Processes
  • 13:27Conclusion

Summary

Automatic Translation

Automatic Translation

We report a device and a new method to study cells and embryos. Single cells are precisely ordered in microcavity arrays. Their 3D confinement is a step towards 3D environments encountered in physiological conditions and allows organelle orientation. By controlling cell shape, this setup minimizes variability reported in standard assays.

Tags

3D ConfinementSingle CellsSingle EmbryosHigh Content ScreeningMicrocavitiesPDMSEgg CupsExtracellular MatrixCell Phenotype3D EnvironmentFission YeastCell SkeletonDrug DiscoveryPersonalized MedicineC. ElegansReplica Molding

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